การระบุยีนที่แสดงออกจำเพาะในระยะยีสต์และสายราของเชื้อ Malassezia furfur

Abstract

Malassezia furfur is a dimorphic fungus found on human skin and warm-blooded animals and sometimes associated with several skin disorders. Generally, the fungus is seen as single-celled yeast but also forms hyphae when they become pathogenic. However, the mechanism of hyphal formation is poorly understood. The purpose of this study was to identify the yeast and hyphal phase-specific genes of M. furfur by cDNA random amplified polymorphic DNA (cDNA-RAPD). According to RAPD fragment pattern analysis, the primers that generated a different banding pattern between the two phases are OPT-01, OPT-02, OPT-05, OPT-06, OPT-07, OPT-08, OPT-09, OPT-13Y1, OPT-17, OPT-18, and OPT-20. Eighteen and two fragments were identified as yeast phase-specific genes and hyphal phase-specific genes, respectively. The yeast phase-specific genes are coding for proteins such as hydrolase, lipid metabolism, ribosome biogenesis, and transmembrane transporter. On the other hand, hyphal phase-specific genes of M. furfur identified as aldo-keto reductase gene and mitochondrial DNA. The present study provides new knowledge for a better understanding of molecular mechanisms of the pathogenicity of M. furfur.