ปริมาณสารสำคัญ ฤทธิ์ทางชีวภาพและเทคนิคการสกัดน้ำมันหอมระเหยจาก ดอกกุหลาบอินทรีย์จากกลุ่มเกษตรกรในอำเภอเชียงดาว จังหวัดเชียงใหม่สู่การพัฒนาผลิตภัณฑ์น้ำหอมและเครื่องสำอาง

Abstract

The Objective of this research was to study and compare the results of the extraction of essential oils from 3 varieties of rose, namely, Mon Dang Prasert, Mon Klai Kangwon and Bishop’s castle which are grown with organic agriculture by farmers in Chiang Dao District, Chiang Mai Province. The ethanol extracts were analysis of the active substance antimicrobial and antioxidant activity. It was found that the ethanol extract of 3 roses sample were all dark red. The percentage yields of Mon Dang Prasert, Mon Klai Kangwon and Bishop’s castle extracts were 9.71, 4.71 and 5.95, respectively. The study of crude extracts from 3 strains of roses against Proteus mirabilis, Escherichia coli, Staphylococcus aureus and Bacillus cereus using Agar well diffusion method was found to inhibit the growth of two gram-positive bacteria, Staphylococcus aureus and Bacillus cereus, and inhibit the growth of one type of Gram-negative bacteria, Proteus mirabilis. All coarse extracts were unable to inhibit the growth of Escherichia coli, a Gram-negative bacteria. The antioxidant activity of Mon Dang Prasert, Mon Klai Kangwon and Bishop’s castle by DPPH method using vitamin C and trolox as standard compounds showed the 〖"IC" 〗_"50" values of 7.4 25.0 30.0 5.5 and 4.3 µg/mL, respectively. Total Phenolic Contents were measured by the folin-chiocalteu method. It was found that the total phenolic content of the Mon Dang Prasert, Bishop’s castle and Mon Klai Kangwon extracts were 606.6 101.93 and 92.31 mg GAE/g extract, respectively. Total flavonoids contents were obtained by the aluminum chloride colorimetric method and found that the Mon Dang Prasert, Mon Klai Kangwon and Bishop’s castle extracts had flavonoid compounds content of 178.76, 42.99 and 38.62 mg QE/g extract, respectively.