การใช้ประโยชน์จากวัสดุเหลือทิ้งจากมะม่วง (เมล็ดในและเปลือกมะม่วง) เพื่อ ใช้เป็นส่วนประกอบของผลิตภัณฑ์เสริมสุขภาพในการลดระดับน้้าตาลในเลือด ของกลุ่มวิสาหกิจชุมชนพัฒนาผลิตภัณฑ์ ต้าบลบ้านโฮ่ง จังหวัดล้าพูน

Abstract

This experimental research is aims to study in vitro and in vivo the pharmacological activities to decrease blood sugar and acute toxicology of mango kernel and peel extraction. The gallic acid and mangiferin were analyzed for basic active constituents by U/HPLC. Five phytochemicals were investigated which are terpenoids, flavonoids, saponins, alkaloids and tannins. DPPH assay and FRAP assay were determined for antioxidant activities. MTT cell proliferation assay were practiced for detection of inhibiting growth of cancer cells. In vitro, the inhibitory effect against the digestive α-amylase and α-glucosidase was determined. In vivo, pharmacological activities to decrease blood sugar and acute toxicology of mango kernel and peel extraction were done in diabetic rats. The results of the study show that 95% ethanol crude extract of mango peel. by stirring (% yield) was 17.19 % while 95% ethanol crude extract of mango kernel by stirring (% yield) was 8.67%. The mango peel extract content of gallic acid was 0.35 ± 0.01 (% w / w) but without Mangiferin. Found that the Gallic acid content was 0.45 ± 0.01 (% w / w) and the Mangiferin content was 0.14 ± 0.01 (% w / w). Crude extract of mango peel were moderate levels of tannins while crude extract of mango kernel were high levels of terpenoids and tannins. The mango kernel extract with 95% Ethanol has the highest total phenolic compound content of 114.22 ± 8.36 mg GAE / g crude extract. 50% Inhibitory Concentration of mango peel and mango kernel by DPPH assay were 198.85 ± 13.40 μg / ml and 194.63 ± 6.46 μg / ml, respectively. The FRAP value of mango peel extract. The seed extracts of mango were 886.15 ± 4.16 μM / mg sample and 1,392.75 ± 2.21 μM / mg sample, respectively. In vitro study of pharmacological activities to decrease blood sugar shown both mango peels and mango kernels extracts had potential to inhibit α-glucosidase ค 4 activities with 43.92 and 19.69times of acarbose. There were potential to inhibit alpha amylase activities with 0.017 and 0.003 times of acarbose. No toxicity was found in the normal cells. In vivo study of pharmacological activities to decrease blood sugar shown mango kernels extract had significant antihyperglycemic effect in diabetic rats when the mango kernels extract was fed 1000 mg/kg. They prevented islet cells damage in diabetic rats. In glucose tolerance, it was found that diabetic rats who were given 1000 mg/kg of kernels extract for 30 days had a significantly reduce in blood sugar levels within 120 minutes. Moreover, it may also increase β-cells function in the pancreas to increase insulinlevel without causing hepatotoxicity and nephrotoxicity in diabetic rats